Department of Biochemistry

Departmental Information:

S.No

Headings

Details

Name of the Department

Biochemistry

Date of Establishment

2017

Name of the HOD

Dr. Manisha Singh

Facility available in Dept.

1. Glucose
2. GTT
3. HbA1c
4. LFT
5. KFT
6. Lipid Profile
7. Electrolytes
8. Amylase& Lipase
9. Iron profile
10. Magnesium
11. Cortisol
12. IL-6
13. BNP
14. Ferritin
15. LDH
16. CRP
17. PCT
18. CK-MB
19. TROP-I
20. Vit. D

Tumour markers

1. CEA
2. CA 125
3. PSA
4. Β HCG
5. Alfa Feto Protein

Hormone analysis

1. Prolactin
2. LH
3. FSH
4. Testosterone
5. Oestrogen
6. Progesterone
7. DHEAS
7. Total T3, T4, TSH

Urinary parameters

1. Micro Albumin
2. 24 hrs Urinary Protein

Facility wise daily work load

1. Teaching of MBBS students including lectures, practical, small group teaching.
2. Running of central biochemistry lab and emergency lab 24/7 with highly trained technicians in hospital.
3. Department faculty members are actively involved in COVID care providing tests like PCT, CRP, IL6, LDH, Ferritin etc. We have handled more than thousand COVID samples in a month.

Training activities & courses run

1. Letter of permission from Medical Council of India vide letter No.
2. MCI-34(41) (E-60)/2019 .Med/ 115574 dated 20th May 2019 for starting MBBS course for 100 students.
3. First batch of 100 students have been admitted and classes has been commenced since 01 Aug 2019.
4. BSc Nurshing Course Teaching
4. DMLT Teaching

Teaching Aids available

LCD Projector
PC available

Services

Central Biochemistry Laboratory

Equipment In Department & Central Biochemistry Labiratory

Equipments or Items

Qty.Avilable

Centrifuge

Dessicator

Flame Photometer

Analytical Balance

Water bath

High speed Homogenizer

Microscope

PH meter

Hot Plate

UV Lamp

Incubator Electric Thermostatic

Digital Colorimeter

Vortex Mixture

Electrophoretic apparatus

Hot air oven

Microwave

Fume Hood

Densitometer With computer (Gel Doc.System )

Glucometer with strip

RT PCR With Accessories

PCR SYSTEM –Thermo cycler with accessories

Deep Refrigerators -20 ͦ C

Orbital shaker

Table Top cold Mini centrifuge

ELISA READER

ELISA WASHER

Dry Bath Incubator

Refrigerators 2- 8 ͦ C

Glass ware

Available

Auto pipette

Available

Semi Autoanalyzer

Thermometers 0-250 C

Thermometers 0-110 C

Bottle dispensers

Spirit Lamps

All Glass Distillation apparatus

Bottle dispensers

Immunoassay analyser (ARCHITECT)

Chemistry analyser (SELECTRA Pro XL)

Chemistry analyser(SELECTRA Pro M)

HBA1C Analyser (D-10)

-20 Horizontal Refrigerator

Vertical Autoclave

Refrigerators

Clinical Centrifuge

Incubator

Inolyte

Cell frost Refrigerator

Chemistry analyser(SELECTRA Pro S)

Semi Auto Analyser

Clinical Remi Centrifuge

ABG Analyser

Cardiac marker Analyser

Mission Statement

The Department of Biochemistry is located on the first floor of Academic Block in the Gautam Budha University campus. The department strives to inculcate the culture of state of art diagnostic laboratory. Our department is well equipped for carrying on undergraduate MBBS course. The diagnostic lab, located in the hospital building is equipped with various autoanlyser providing a plethora of tests available 24 hrs , required in patient care.

Mission:

. Provide excellent teaching and learning environment for students.
. To provide a wide range of test panel for patients.
. Establishment of lab for inborn errors of metabolism.

Research Projects by the Department Biochemistry:

1.Implementation of 5S management method for lean health care in clinical biochemistry laboratory of a government hospital in Uttar Pradesh, India
2. Implementation of Flipped Classroom Model in teaching Biochemistry to Phase – I MBBS students.
3. Relationship between Telomere length, inflammatory markers, Endothelin- 1 and oxidative stress in preeclampsia
4. To explore any association of vitamin D3 with laboratory parameter in COVID 19 patients preeclampsia
5. Evaluation of laboratory parameter for diagnostic accuracy in RTPCR positive COVID 19 patients
6. COVID 19 infection in pregnancy analysis of potential feto maternal effects and risk of vertical transmission.
7. Role of short term oral estradiol in natural estrogen deficient symptomatic COVID 19 patients- a RCT.
8. To study the predictors of disease severity in COVID 19 infected patients in a tertiary care center in Greater Noida.

CME/Workshops organized by the department

A CME cum Workshop was organized on Advanced Molecular Techniques in Medical Diagnostics on 22nd & 23rd April 2019. This event had been conceived with the idea of providing platform for amalgamation of modern technologies in diagnosis of different disorders. The CME cum Workshop offered a wonderful mix of high caliber plenary speakers, an engaging scientific program and interesting talks on “Advanced molecular techniques in medical diagnostics”.

NABL training program was conducted for 2 days on 11th and 12th December 2019.
Conducted guest lecture on “Cell Culture in Medical Research- current trends and future perspectives”, given by Prof B C Koner, HOD, Dept. of Biochemistry, MAMC, New Delhi.

Departmental Achievements:

Improved the Laboratory infrastructure by including new analyzers and providing a good panel of tests for patient care.
“Certificate of Accreditation” for Central Biochemistry Laboratory from National Accreditation Board for testing and Calibration Laboratories under ISO 15189:2012 Certificate Number MC- 4307

VISION PLAN OF DEPARTMENT OF BIOCHEMISTRY

Present status

First 1 year

First 5 years

Next 10 years

*MBBS Teaching.

*To get permission for Post Graduate MD seats

*Upgrading Research

Centre of excellence in Diagnostic, Training & teaching.

*Diagnostic Laboratory.

*Training of all staff members according to NABL standards.

*Screening of Inborn Errors of Metabolism

*To facilitate research and get extramural projects sanctioned

*Electrophoresis & Immunofixation for multiple Myeloma.

Organising CMEs & Workshops

To start PhD

Road map for next 10 years:

1. Develop the department as a Centre of excellence for undergraduate & postgraduate training.
2. To provide state of art diagnostic facility in Gautham Buddha University
3. To improvise the research in the department.

Work Load Of Central Biochemistry Laboratory (Year 2021-2022)

FACULTY

Papers published/ read by faculty

ACADEMIC AWARDS/ ACHIEVEMENTS:

1. Best Teacher Award 2021
2.Advanced Course in Medical Education from CMC Ludhiana.
3. Organising Secretary of CME-cum-Workshop on “Advanced Molecular Techniques in Medical Diagnostics” held at GIMS
4. Resource person in the Revised Basic Course (RBC) on Medical Education and ATCOM held at GIMS
5. Certificate course of CISP I & II in Medical Education
6. Basic course in Biomedical Research by ICMR
7. Certificate course in International Conference on Harmonization - Good Clinical Practice(ICH-GCP).
8. Certificate in Quality Management System and Internal Audit in Medical Labs as per ISO 15189:2012
9. Certificate course on Principles of Bioethics and Human rights

Organized Academic Sessions:

A CME cum Workshop was organized on Advanced Molecular Techniques in Medical Diagnostics on 22nd& 23rd April 2019. This event had been conceived with the idea of providing platform for amalgamation of modern technologies in diagnosis of different disorders. The CME cum Workshop offered a wonderful mix of high caliber plenary speakers, an engaging scientific program and interesting talks on “Advanced molecular techniques in medical diagnostics”.

NABL training program was conducted for 2 days on 11th and 12th December 2019.

Conducted guest lecture on “Cell Culture in Medical Research- current trends and future perspectives”, given by Prof B C Koner, HOD, Dept. of Biochemistry, MAMC, New Delhi.

PAPER PUBLICATIONS

1.Implementation of 5S management method for lean health care in clinical biochemistry laboratory of a government hospital in India
2.Dr. Manisha Singh¹, Dr. Mamta Padhy², Dr. Deepti Chopra³.Implementation of Flipped Classroom Model in teaching Biochemistry to Phase-I MBBS Students. European Journal of Molecular & Clinical Medicine (EJMCM), 2022; 09(03),2515-
3.Ravoori Saideswar Rao, Manisha Singh, Ritu Sharma, Mamta Padhy, Preeti Sharma, Rakesh Gupta, Maternal circulatory levels of procalcitonin, uric acid, and lipid profile in Early-onset preeclampsia and Late-onset preeclampsia. International Journal of Health Sciences, 6(S5), 1543-1550.
4. Padhy M, Garg AK, Solanki HK, Rao R S, Gupta V, Singh M. A cross-sectional study to explore the correlation of serum Vitamin D3 with laboratory parameters among COVID-19 patients of a tertiary care centre of Uttar Pardesh, India. International Journal of health and clinical research, 2021;Vol 4(22): 181-187
5.Padhy M, Garg AK, Solanki HK, Rao R S, Gupta V, Singh M. Evaluating laboratory parameters for diagnostic accuracy in COVID 19 patients in a tertiary care hospital in Greater Noida, Up, India. International Journal of health and clinical research, 2021;4(18):288-299
6. Seth S, Sharma R, Mishra P, Solanki HK, Singh M, Singh M. Role of short term Estradiol supplementation in symptomatic postmenopausal COVID !9 females:A randomized controlled trial. J Mid-life Health 2021;12;211-8
7. Singh M, Karli S, Choudhary S. Fetuin- A in association with metabolic syndrome. Int J Res Med Sci.2021: 9(8);2342-46.
8.Rachna Kalani, Anu Chandra, Shipra Kunwar, Manisha Singh, Aparna Misra. Thyroid Stimulatory Hormone (TSH) Reference ranges in the normal pregnant women of Lucknow. Eras Journal of Medical Research.2018;5(2)
9. Manisha Singh, Eqbal Anwar, Vishnu Kumar. Assessment of biochemical parameters in the patients of coronary artery disease with Type 2 Diabetes Mellitus. International Journal of Pharmaceutical Sciences and Research(2017); Vol8, Issue 3
10. Asfia Khan, BrijeshRathore, Manisha Singh,AparnaMisra. Association of creatine kinase and its isoenzymes(CK-MB, CK-BB) activity with high risk pregnancy. International Journal of Research in Medical Sciences. 2017, Apr;5(4):1464-1470
11. Aparna Misra, Manisha Singh, Anchal Trivedi, Brijesh Singh Rathore, Anil K. Balapure. In-vitro effect of Picroliv on Plasmodium Berghei Induced Alterations in the Activity of Adenosine Triphosphatase, Aryl Hyrocarbon Hydroxylase Enzymes and MDA in Liver Explant Culture. Indian Journal of Pathology Research and Practice, Volume 5 Number 2, May-August 2016.
12. Manisha Singh, IpsitaChoudhary, VasanthiPallinti, R Jothimalar, Visnhu Kumar and Aparna Misra. Association between PRO12ALA polymorphism at the PPAR?2 gene and insulin sensitivity in South Indian population with type-2 diabetes mellitus. Indian Journal of Basic and Applied Medical Research; June 2016: Vol-5, Issue-3, P. 722-741
13. BrijeshRathore, Manisha Singh, Vishnu Kumar, and Aparna Misra. Osteocalcin: An Emerging Biomarker for Bone Turnover. International Journal of Research in Medical Sciences (2016) 4 (9): 3670-3674.
14. Vishnu Kumar, RituKaroli, Manisha Singh, Aparna Misra and Farzana Mahdi. Evaluation of Oxidative Stress, Antioxidant Enzymes, Lipid and Lipoprotein Profile in Type-2 Diabetic Patients. International Journal of Bioassays: September 6, 2015; Accepted: September 25, 2015
15. Vishnu Kumar, Farzana Mahdi, Ranjana Singh, Abbas Ali Mahdi, Kalbe Jawad, Raj Kumar Singh and Manisha Singh (2015). Cassia Tora Seeds Extract Regulates Lipid Metabolism in Alloxan Induced Diabetic Rats. International Journal of Pharmacology Science and Research Vol. 6(8): 3484-3489.
16. RichaGupta, Manisha Singh, JibhateAnirudha, Mahdi Farzana.Novel Methodology for Implementation of OSCE as Examination Tool to Delimit Space and Time Constraints. Journal of Medical Education& Research, Vol 3, No.1,Jan June 2011

ACADEMIC AWARDS/ ACHIEVEMENTS:

● Masters of Science in Clinical Research and Regulatory Affairs from Sikkim Manipal University
● Certificate course in Diabetology from PHFI
● MBA in International Business from ICFAI, Hyderabad
● Co-authored a book titled “ Medical Bioinformatics and Biochemistry [Diabormatics]”
● Successfully Completed “Revised Basic Course Workshop in Medical Education Technology”
● Successfully Completed the XXIX Internal Audit and Quality Management Systems Certificate Course
● Conducted MBBS, BDS University examination in the capacity of Internal Examiner.
● Had been appointed as Paper Setter for MBBS, BDS and MD examination of various Government as well as Private Medical Colleges & Universities, especially in Assam and across India.
● Had been invited as an External Examiner for evaluation of scripts of PG and UG courses of various Government as well as Private Medical Colleges & Universities, especially in Assam and Delhi NCR.
● Had been invited as an External examiner for conducting MD, PhD, MBBS and BDS University examination in various Government as well as Private Medical Colleges & Universities since 2012, especially in Assam, Delhi NCR and across India.
● Conducted MCI Inspections successfully as Assessor.
● Has participated and chaired many scientific sessions/ conferences and lectures in various symposium and Workshop held throughout the country (ACBICON in NIMHANS, Bangalore , AMBICON in Shillong and Assam etc.)

OTHER ACHIEVEMENTS

● Incharge of various Training Activities and Sessions during COVID19 pandemic period.
● Attended Video Conferences from CM and Health Ministries of UP during COVID19 pandemic period.
● Awarded Certificate of Excellence from ASMC , Hardoi, UP
● Publications in Indexed Journals – 22 ( 14 as 1st / 2nd Author)
⮚ National – 6
⮚ International – 16

PAPER PUBLICATIONS

▪ BHATTACHARYA I, PRAVEENA V, SAXENA R. ; STUDY ON DYSLIPIDAEMIA AND OXIDATIVE STRESS IN ELDERLY – A CLINICAL APPROACH TO PREDICT CARDIOVASCULAR DISEASE RISK. ( AUTHORS – KUMAR VS, ; SCHOLARS JOURNAL OF APPLIED MEDICAL SCIENCES. 2017 ; 5(4) : 1504-1508.

▪ FALDESAI N, SHAREEF MA, SAXENA R, BHATTACHARYA I. ; ASSOCIATION OF VITAMIN C WITH ERYTHROCYTE MALONDIALDEHYDE LEVELS IN TYPE 2 DIABETIC PATIENTS – A CLINICAL APPROACH. ; SCHOLARS JOURNAL OF APPLIED MEDICAL SCIENCES. 2017 ; 5(11D) : 4606-4610

▪ PRAJAPAT R, BHATTACHARYA I. ; EFFECT OF VITAMIN E AND C SUPPLEMENTATION ON OXIDATIVE STRESS IN DIABETIC PATIENTS. ; ADVANCES IN DIABETES AND METABOLISM. 2017. 5(3), 39-42, DOI:10.13189/ADM.2017.050301

▪ PRAJAPAT R, BHATTACHARYA I, JAKHALIA A. ; COMBINED EFFECT OF VITAMIN C AND E DOSE ON TYPE 2 DIABETES PATIENTS. ; ADVANCES IN DIABETES AND METABOLISM. 2017. 5, 21-25, DOI:10.13189/ADM.2017.050201

▪ SHAREEF MA, FALDESAI N, SAXENA R, BHATTACHARYA I. ; ASSESSMENT OF PLASMA PARAOXONASE ACTIVITY IN METABOLIC SYNDROME PATIENTS. ; SCHOLARS JOURNAL OF APPLIED MEDICAL SCIENCES. 2018 ; 6(2) : 609-613.

▪ KARKI K, AGARWAL S, RAI G, SAXENA R, SRIVASTAVA S, SHRESTHA S, BHATTACHARYA I. ; ASSOCIATION OF OXIDATIVE STRESS AND PSYCHOLOGICAL STRESS IN HYPERTENSIVE AND NORMOTENSIVE DIABETES PATIENTS. ; SCHOLARS JOURNAL OF APPLIED MEDICAL SCIENCES. 2019 ; 7(7) : 2504-2507.

▪ KUMAR A, KAPOOR S, BHATTACHARYA I ; TO COMPARE THE LEVELS OF VITAMIN B12, FOLATE AND FERRITIN WITH THYROID HORMONES IN HYPOTHYROIDISM PATIENTS: AN INSTITUTIONAL BASED STUDY. ; INTERNATIONAL JOURNAL OF MEDICAL RESEARCH PROFESSIONALS. 2020, P-ISSN: 2454-6356; E-ISSN: 2454-6364. DOI: 10.21276/ijmrp

ACADEMIC AWARDS/ ACHIEVEMENTS:

1. Completed Diploma in Hospital Administration from National Institute of Health and Family welfare
2. MHA from Academy of Hospital Administration
3. Joint Organizing Secretary of CME-cum-Workshop on “Advanced Molecular Techniques in Medical Diagnostics at GIMS
4. Certificate course in Basic Revised Workshop
5. Certificate course in Biomedical Research by ICMR
6. Training in Molecular techniques in RTPCR in IGIB

Organized Academic Sessions:

NABL training program was conducted for 2 days on 11th and 12th December 2019.

Examinership: Examiner for BMLT and MSc at Jamia Hamdard University

Other Achievements:

1. Certificate of appreciation in Molecular and Diagnostic and Research Laboratory
2. Diploma in Hospital Administration from National Institute of Health and
3. Family welfare in 2018
4. MHA from Academy of Hospital Administration, Noida
5. Internal Auditor as per ISO 15189:2012 (NABL)
6. Certificate course in Basic Revised WorkshopCertificate course in Biomedical Research by ICMR
7. Quality Manager -NABL

PAPER PUBLICATIONS

1.Implementation of 5S management method for lean health care in clinical biochemistry laboratory of a government hospital in India
2.Dr. Manisha Singh¹, Dr. Mamta Padhy², Dr. Deepti Chopra³.Implementation of Flipped Classroom Model in teaching Biochemistry to Phase-I MBBS Students. European Journal of Molecular & Clinical Medicine (EJMCM), 2022; 09(03),2515-
3.Ravoori Saideswar Rao, Manisha Singh, Ritu Sharma, Mamta Padhy, Preeti Sharma, Rakesh Gupta, Maternal circulatory levels of procalcitonin, uric acid, and lipid profile in Early-onset preeclampsia and Late-onset preeclampsia. International Journal of Health Sciences, 6(S5), 1543-1550.
4. Padhy M, Garg AK, Solanki HK, Rao R S, Gupta V, Singh M. A cross-sectional study to explore the correlation of serum Vitamin D3 with laboratory parameters among COVID-19 patients of a tertiary care centre of Uttar Pardesh, India. International Journal of health and clinical research, 2021;Vol 4(22): 181-187
5.Padhy M, Garg AK, Solanki HK, Rao R S, Gupta V, Singh M. Evaluating laboratory parameters for diagnostic accuracy in COVID 19 patients in a tertiary care hospital in Greater Noida, Up, India. International Journal of health and clinical research, 2021;4(18):288-299
6. Serum Neutrophil Gelatinase Associated Lipocalin(NGAL) and Cystatin C) as early predictors of Contrast-Induced Acute Kidney Injury in patients undergoing percutaneous coronary ClinicaChimica Acta 435 (2014) 48-52.
7. Correlation of oxidative stress biomarker and serum marker of brain injury in hypoxic ischemic encephalopathy International Journal of Medical & Applied Sciences,Volume 3,Issue 1,2014
8. Procalcitonin and C-reactive protein in early diagnosis of neonatal sepsis Indian Journal of Medical Specialities.Print-0976-2884.Online-0976-2892

Dr. Devesh Sharma

Assistant Professor

Dr. Devesh Sharma did his MBBS from Kasturba Medical College, Manipal and completed MD Biochemistry from University College of Medical Sciences, Delhi. He has worked earlier in AIIMS, New Delhi, VMMC and Safdarjung Hospital and Delhi State Cancer Institute, Govt of NCT of Delhi. His areas of interest includes cancer biomarkers, Type 2 diabetes and medical education.

Academic Award/Achievements:

1. First prize in oral presentation at Indian Society of Atherosclerosis Research – Delhi chapter conference in July 2017.
2. Basic Course in Biomedical Research by ICMR
3. Certificate in Quality Management System and Internal Audit in Medical Labs as per ISO 15189:2012
4. KVPY research fellowship finalist.
5. Trained in Tumour markers reporting at Delhi State Cancer institute.
6. Implementing the CBME curriculum through ECEs, Problem based Learning ( SDL) , Roleplays, Small group discussions.
7. Initialising the biochemistry lab in the new emergency block at Safdarjung hospital.
8. Topper in MD Biochemistry exam (Delhi University) held in 2017.

PAPER PUBLICATIONS

1. Relationship of serum apolipoprotein AV levels, oxidative stress and inflammatory biomarkers with hypertriglyceridemia in type 2 diabetes mellitus D Sharma, S Garg, M Mehndiratta, SV Madhu, D Puri International Journal of Endocrinology and Metabolism 15 (2)
2. Benign Ovarian Cysts with Raised CA-125 Levels: Do We Need to Evaluate the Fallopian Tubes? D Sharma, Vinocha Journal of laboratory physicians 12 (04), 276-280
3. Laboratory Changes in SARS-CoV-2 Infection: A Review D Sharma, M Mehndiratta, D Puri. Indian Journal of Medical Biochemistry 24 (2), 63
4. Association of apolipoprotein AV with mRNA expression of IL-6 and NF-κB genes in type 2 diabetes with hypertriglyceridemia: a possible link with inflammation D Sharma, S Garg, M Mehndiratta, SV Madhu, D Puri International Journal of Diabetes in Developing Countries 39 (2), 308-314
5. Diabetes with Complication-scope of Serum Ceruloplasmin as a Biomarker K Prabhu, D Sharma, VS BELLE, M SUNDARAM, P GYAWALI, Journal Of International Academic Research For Multidisciplinary 2 (5), 841-6
6. Expression profile of NFKB1 gene in the patients of type II diabetes with and without hypertriglyceridemia. M Mehndiratta, S Garg, D Sharma, SV Madhu, D Puri Indian Journal of Endocrinology & Metabolism 21

Miss Surabhi Puri

PAPER PUBLICATIONS

1.Parul Gupta Surabhi Puri Effect of Ajowan Seed Powder in Modulating Hyperglycemia Induced Oxidative Stress in Normal and NIDDM Patients- A Prospective Study Volume : 5 | Issue : 11 | November 2016 • ISSN No 2277 - 8179 | IF : 3.508 | IC Value : 69.48
2. Puri S, Chaudhary I, Anand P, Singh UN. Correlation of vitamin D with different trimesters of pregnancy.

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RESIDENTS/ TUTORS IN DEPARTMENT OF BIOCHEMISTRY

Non-Teaching Staff of Department of Biochemistry:

Non Teaching Staff Details For Central Lab:

Departmental Information:

Tumour markers

Hormone analysis

Urinary parameters

Equipment In Department & Central Biochemistry Labiratory

Mission Statement

Mission:

Research Projects by the Department Biochemistry:

CME/Workshops organized by the department

Departmental Achievements:

VISION PLAN OF DEPARTMENT OF BIOCHEMISTRY

Road map for next 10 years:

Work Load Of Central Biochemistry Laboratory (Year 2021-2022)

FACULTY

Papers published/ read by faculty

Dr. Manisha Singh

ACADEMIC AWARDS/ ACHIEVEMENTS:

Organized Academic Sessions:

PAPER PUBLICATIONS

Dr. Ijen Bhattacharya

ACADEMIC AWARDS/ ACHIEVEMENTS:

OTHER ACHIEVEMENTS

PAPER PUBLICATIONS

Dr. Mamta Padhy

ACADEMIC AWARDS/ ACHIEVEMENTS:

Organized Academic Sessions:

Other Achievements:

PAPER PUBLICATIONS

Dr. Devesh Sharma

Academic Award/Achievements:

PAPER PUBLICATIONS

Miss Surabhi Puri

PAPER PUBLICATIONS

Department Pics

DEPARTMENT OF BIOCHEMISTRY

DEMO ROOM

UG Laboratory

Departmental Library

ELISA STATION

Biochemistry Central Laboratory

Biochemistry Workshop

Biochemistry Workshop